山羊多克隆抗体to Prostatic Acid Phosphatase
Mouse, Rat, Cow
, corresponding to internal sequence amino acids 247-259 of Human Prostatic Acid Phosphatase (NP_001090.2).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
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Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 45 kDa.
||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the histidine acid phosphatase family.
Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Information by UniProt
- 5'-NT antibody
- 5'-nucleotidase antibody
- Acid phosphatase prostate antibody
Western blot - Anti-Prostatic Acid Phosphatase antibody (ab103893)
Anti-Prostatic Acid Phosphatase antibody (ab103893) at 0.3 µg/ml + Human Prostate Lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostatic Acid Phosphatase antibody (ab103893)
ab103893 (3.8µg/ml) staining of paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
has not yet been referenced specifically in any publications.
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