Anti-Prostate Specific Antigen抗体(ab53774)


  • 产品名称Anti-Prostate Specific Antigen抗体
    参阅全部 Prostate Specific Antigen 一抗
  • 描述
    兔多克隆抗体to Prostate Specific Antigen
  • 特异性This antibody detects endogenous levels of total Prostate Specific Antigen protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from human Prostate Specific Antigen

  • 阳性对照
    • Extracts from mouse heart



Our Abpromise guarantee covers the use of ab53774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • 功能Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • 序列相似性Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody 图像

  • All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution

    Lane 1 : Extracts from mouse heart
    Lane 2 : Extracts from mouse heart, plus immunising peptide

    Predicted band size : 29 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Prostate Specific Antigen antibody (ab53774)参考文献

This product has been referenced in:
  • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). Read more (PubMed: 25680117) »
  • Rodríguez-Lorenzo L  et al. Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. Nat Mater 11:604-7 (2012). Human . Read more (PubMed: 22635043) »

See all 2 Publications for this product

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Thank you for contacting us.

Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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Thank you for contacting Abcam.

Theimmunogen sequence for ab53774is from ****** of human Prostate Specific Antigen.

We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made...

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You are correct that ab39983 has been discontinued. I am sorry about that. I should have checked all products were still available. Here are 2 more product recommendations for your customer: Anti-Prostate Specific Antigen antibody (ab537...

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Thank you for your enquiry. Based on the researcher's notes, I'm not quite sure what the customer is interested in doing with their experiment. But regarding your question, I don't think ab53774 will react with the PSA peptide ab45800. As noted ...

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