Anti-Prostate Specific Antigen抗体[8A6] (ab10187)

Submit an Abreview Q&A (5)Specific References (2)

概述

  • 产品名称
    Anti-Prostate Specific Antigen抗体[8A6]
    参阅全部 Prostate Specific Antigen 一抗
  • 描述
    小鼠单克隆抗体[8A6] to Prostate Specific Antigen
  • 宿主
    Mouse
  • 特异性
    Specific for free PSA, epitope 1. No cross reactivity with human kallikrein 2.
  • 经测试应用
    适用于: Sandwich ELISA, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human PSA antigen purified from semenal plasma.

  • 常规说明

    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab76113 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab10187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution. Best pair for detection of free PSA - (detection ab - capture ab) ab10187 - ab10185 For the detection of free PSA ab10187 can be used as a detection antibody in conjugation with ab10184. For the detection of free PSA ab10187 can be used as a detection antibody in conjugation with ab24466.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • 序列相似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    文献

    This product has been referenced in:
    • Xie C & Wang G Development of simultaneous detection of total prostate-specific antigen (tPSA) and free PSA with rapid bead-based immunoassay. J Clin Lab Anal 25:37-42 (2011). Read more (PubMed: 21254241) »
    • Escamilla-Gómez V  et al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron 24:2678-83 (2009). Human . Read more (PubMed: 19261459) »

    See all 2 Publications for this product

    发表研究结果有使用 ab10187？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    5 reviews or Q&A

    10
    Votes: Highest First

    I would like to use ab10185 and ab10187 in a sandwich ELISA. Will they work in the following combinations:

    ab10185 (capture) - ab10187 (detection)
    ab10187 (capture) - ab10185 (detection)

    Yes, it is possible to use this pair in either combination.

    Dear Technical Services,



    I would like to know if these antibody formulation contain BSA:



    1. Ab10186 clone # 5G6

    2. Ab10187 clone# 8A6

    3. Ab10184 clone# 2H9

    4. Ab1...

    Read More

    Thank you for contacting us. None of these products contain BSA. We do have the storage buffer listed on the datasheets for your convenience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

    Read More

    Dear technical team,

    Our customer purchased ab10187, ab10184, ab10185, ab119154 for sELISA.
    Her major is chemistry and she is going to apply sELISA application to her experiment.
    So she wants sELISA data of these antibodies.
    I...

    Read More

    Thank you for your enquiry.

    We have used our internal sandwich ELISA protocol only to determine which monoclonal antibodies are working together as pairs. Usually any general protocol, like for example the one youfind below, with proper opti...

    Read More

    Thanks for your kindly reply; this customer would like to conduct the non-conventional application which process is similar to ELISA application. I attached the purpose image in this letter; this customer wants to know whether this experiment can work ...

    Read More

    Thank you for your reply.

    I have reviewed the details of this experiment and have the following feedback:

    1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

    Read More

    How was this antibody shown to be specific for free PSA and not PSA in complex?

    Thank you for your enquiry. Specificity was tested in ELISA with free PSA or PSA-ACT complex as antigen. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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