Anti-Prostate Specific Antigen抗体[5A6] (HRP) (ab24466)

概述

  • 产品名称
    Anti-Prostate Specific Antigen抗体[5A6] (HRP)
    参阅全部 Prostate Specific Antigen 一抗
  • 描述
    小鼠单克隆抗体[5A6] to Prostate Specific Antigen (HRP)
  • 偶联物
    HRP
  • 特异性
    Equimolar total PSA, epitope 5. No cross reactivity for human kallikrein 2.
  • 经测试应用
    适用于: Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human PSA antigen purified from semenal plasma).

  • 阳性对照
    • This antibody gave a postive signal in Human Prostate tissue lysate and LNCaP whole cell lysate.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab24466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187).

For the detection of free PSA, ab24466 can be used as a detection antibody in conjugation with ab10187. For total PSA detection ab24466 can be used as a capture antibody and ab10184 as a detection antibody.

WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • 序列相似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

图片

  • All lanes : Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466) at 1/5000 dilution

    Lane 1 : Human prostate tumor tissue lysate (adenocarcinoma) - total protein (ab30305)
    Lane 2 : LNCaP nuclear extract lysate (ab14857)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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Thank you for contacting Abcam.

It appears our datasheet for ab24466 incorrectly states that 0.1% sodium azide is included in the storage buffer. Sodium azide is not present in the storage buffer since, as you mentioned, sodium azide affects H...

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That is correct: ab10184 for capture and ab24466 for detection. These are two different clones.  The detection antibody, ab24466, is conjugated to HRP. Some of the catalogue numbers of our other PSA antibodies are very similar so I will not even mentio...

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Thank you for your inquiry. Unfortunately, the isoelectric points of these antibodies have not been determined. According to my research, the pI of most monoclonal antibodies is around 6.1. The isoelectric point for horseradish peroxidase isozyme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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