Anti-Prostate Specific Antigen抗体[5A6] (HRP) (ab24466)

概述

  • 产品名称Anti-Prostate Specific Antigen抗体[5A6] (HRP)
    参阅全部 Prostate Specific Antigen 一抗
  • 描述
    小鼠单克隆抗体[5A6] to Prostate Specific Antigen (HRP)
  • 偶联物HRP
  • 特异性Equimolar total PSA, epitope 5. No cross reactivity for human kallikrein 2.
  • 经测试应用适用于: Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human PSA antigen purified from semenal plasma).

  • 阳性对照
    • This antibody gave a postive signal in Human Prostate tissue lysate and LNCaP whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab24466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187).

For the detection of free PSA, ab24466 can be used as a detection antibody in conjugation with ab10187. For total PSA detection ab24466 can be used as a capture antibody and ab10184 as a detection antibody.

WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • 序列相似性Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody [5A6] (HRP) 图像

  • All lanes : Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466) at 1/5000 dilution

    Lane 1 : Human prostate tumor tissue lysate (adenocarcinoma) - total protein (ab30305)
    Lane 2 : LNCaP nuclear extract lysate (ab14857)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466)参考文献

ab24466 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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Thank you for contacting Abcam.

It appears our datasheet for ab24466 incorrectly states that 0.1% sodium azide is included in the storage buffer. Sodium azide is not present in the storage buffer since, as you mentioned, sodium azide affects H...

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That is correct: ab10184 for capture and ab24466 for detection. These are two different clones.  The detection antibody, ab24466, is conjugated to HRP. Some of the catalogue numbers of our other PSA antibodies are very similar so I will not even mentio...

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Thank you for your inquiry. Unfortunately, the isoelectric points of these antibodies have not been determined. According to my research, the pI of most monoclonal antibodies is around 6.1. The isoelectric point for horseradish peroxidase isozyme...

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