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Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.
Our Abpromise guarantee covers the use of ab10185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [2H9] to PSA (ab10184), and Mouse monoclonal [8A6] to PSA (ab10187).|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187). best pair for the detection of free PSA: (detection ab - capture ab) ab10187 - ab10185 For the detection of total PSA ab10185 can be used as capture antibody in conjunction with ab10184. For the detection of total PSA ab10185 can be used as a detection antibody in conjugation with ab10186.|
ab10185 has not yet been referenced specifically in any publications.