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Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.
Concentration varies from lot to lot and can be provided on request.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab76113 as a replacement.
Our Abpromise guarantee covers the use of ab10185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [2H9] to PSA (ab10184), and Mouse monoclonal [8A6] to PSA (ab10187).|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187). best pair for the detection of free PSA: (detection ab - capture ab) ab10187 - ab10185 For the detection of total PSA ab10185 can be used as capture antibody in conjunction with ab10184. For the detection of total PSA ab10185 can be used as a detection antibody in conjugation with ab10186.|
ab10185 has not yet been referenced specifically in any publications.