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Human PSA antigen purified from semenal plasma.
Our Abpromise guarantee covers the use of ab10184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187) and Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).
Detection of PSA antigen. Recommended pairs for detection of total PSA (capture - detection):
ab10184 (capture) - ab10185 (detection)
ab24466 (capture) - ab10184 (detection)
Recommended pairs for detection of free PSA (capture - detection):
ab10184 (capture) - ab10187 (detection)
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
ICC/IF image of ab10184 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10184, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.