Anti-Prostate Secretory Protein/PSP抗体[YPSP-1] (ab19070)

概述

  • 产品名称Anti-Prostate Secretory Protein/PSP抗体[YPSP-1]
    参阅全部 Prostate Secretory Protein/PSP 一抗
  • 描述
    小鼠单克隆抗体[YPSP-1] to Prostate Secretory Protein/PSP
  • 特异性Does not react with a variety of human normal tissues.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (purified) (Human).

  • 阳性对照
    • Reactive with PSP or human prostate epithelial cells.

性能

应用

Our Abpromise guarantee covers the use of ab19070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a dilution of 1/100.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder.
    • 疾病相关Defects in MSMB are the cause of susceptibility to prostate cancer hereditary type 13 (HPC13) [MIM:611928]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    • 序列相似性Belongs to the beta-microseminoprotein family.
    • 细胞定位Secreted. Sperm surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta microseminoprotein [Precursor] antibody
      • Beta-microseminoprotein antibody
      • HPC 13 antibody
      • HPC13 antibody
      • IGBF antibody
      • Immunoglobulin binding factor antibody
      • Immunoglobulin-binding factor antibody
      • microseminoprotein beta antibody
      • Msmb antibody
      • MSMB_HUMAN antibody
      • MSP antibody
      • MSPB antibody
      • PN 44 antibody
      • PN44 antibody
      • Prostate secreted seminal plasma protein antibody
      • Prostate secretory protein of 94 amino acids antibody
      • Prostate secretory protein PSP94 antibody
      • PRPS antibody
      • PRSP antibody
      • PSP 57 antibody
      • PSP antibody
      • PSP-94 antibody
      • PSP57 antibody
      • PSP94 antibody
      • Seminal plasma beta inhibin antibody
      • Seminal plasma beta-inhibin antibody
      see all

    Anti-Prostate Secretory Protein/PSP antibody [YPSP-1] 图像

    • Anti-Prostate Secretory Protein/PSP antibody [YPSP-1] (ab19070) at 1 µg/ml + Prostate Tumor Lysate Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 14 kDa
      Observed band size : 14 kDa
      Additional bands at : 45 kDa,54 kDa,74 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes

    Anti-Prostate Secretory Protein/PSP antibody [YPSP-1] (ab19070)参考文献

    This product has been referenced in:
    • Whitaker HC  et al. The potential value of microseminoprotein-beta as a prostate cancer biomarker and therapeutic target. Prostate 70:333-40 (2010). Read more (PubMed: 19790236) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (LNCaP Prostate cell line)
    Specification LNCaP Prostate cell line
    Fixative Acetone
    Permeabilization Yes - 0.1% Triton X 100
    Blocking step Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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    Verified customer

    提交于 Apr 01 2008

    The originator of this antibody was able to provide the following information regarding the antibody's use in ELISA. The immunogen was prepared from serum (for the preparation method, please refer to: Int. J. Cancer (1990) 46:39-49) and the antibody wa...

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    Thank you for your enquiry and patience. I'm trying to obtain more information from the originator of this product and will let you know when I do. I did a search and did not find a commercial source for purified PSP. Also recommended as a positive con...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"