Anti-Prostaglandin I2 Receptor抗体(ab60706)

概述

  • 产品名称Anti-Prostaglandin I2 Receptor抗体
    参阅全部 Prostaglandin I2 Receptor 一抗
  • 描述
    小鼠单克隆抗体to Prostaglandin I2 Receptor
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment with tag: RKAVFQRLKL WVCCLCLGPA HGDSQTPLSQ LASGRRDPRA PSAPVGKEGS CVPLSAWGEG QVEPLPPTQQ SSGSAVGTSS KAEASVACSL C, corresponding to amino acids 296-387 of Human Prostaglandin I2 Receptor

  • 阳性对照
    • A549 whole cell lysate and human tonsil tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab60706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-P
WB
ICC/IF
  • 应用说明ELISA: Use at an assay dependent dilution.
    Note: The detection limit for ab60706 is approximately 0.1ng/ml as a capture antibody.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 41 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    • 翻译后修饰Palmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.
      Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • IP antibody
      • PGI receptor antibody
      • PGI2 receptor antibody
      • PI2R_HUMAN antibody
      • PRIPR antibody
      • Prostacyclin receptor antibody
      • Prostaglandin I2 (prostacyclin) receptor (IP) antibody
      • Prostaglandin I2 receptor antibody
      • Prostanoid IP receptor antibody
      • Ptgir antibody
      see all

    Anti-Prostaglandin I2 Receptor antibody 图像

    • ab60706 at 3µg/ml staining Prostaglandin I2 Receptor in human tonsil by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
    • Anti-Prostaglandin I2 Receptor antibody (ab60706) + A549 whole cell lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG HRP at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 41 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Anti-Prostaglandin I2 Receptor antibody (ab60706)参考文献

    This product has been referenced in:
    • Larsson-Callerfelt AK  et al. Defective alterations in the collagen network to prostacyclin in COPD lung fibroblasts. Respir Res 14:21 (2013). ICC/IF ; Human . Read more (PubMed: 23406566) »

    See 1 Publication for this product

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