Anti-Prostaglandin E2抗体(ab2318)

概述

  • 产品名称
    Anti-Prostaglandin E2抗体
    参阅全部 Prostaglandin E2 一抗
  • 描述
    兔多克隆抗体to Prostaglandin E2
  • 经测试应用
    适用于: ELISA, IHC-P, ICCmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Highly pure Prostaglandin E2 conjugated to bovine thyroglobulin

  • 阳性对照
    • Purified PGE2

性能

应用

Our Abpromise guarantee covers the use of ab2318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000 - 1/100000.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration. PubMed: 23762835

靶标

  • 相关性
    Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis.
  • 别名
    • PG E2 antibody
    • PGE 2 antibody
    • PGE2 antibody
    • ProstaglandinE2 antibody

Anti-Prostaglandin E2 antibody (ab2318)参考文献

This product has been referenced in:
  • Ho ATV  et al. Prostaglandin E2 is essential for efficacious skeletal muscle stem-cell function, augmenting regeneration and strength. Proc Natl Acad Sci U S A 114:6675-6684 (2017). IHC ; Mouse . Read more (PubMed: 28607093) »
  • Esbona K  et al. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res 18:35 (2016). IHC-P . Read more (PubMed: 27000374) »

See all 10 Publications for this product

Product Wall

Thank you very much for your interest in ab2318 Anti-Prostaglandin E2 antibody.

To our knowledge, ab2318 has still not been tested in a cell culture assay due to the sodium azide. As my collegue suggest it is possible to extract the chemical...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
Permeabilization
Yes - 0.5% Tween 20
Specification
spleen
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 27 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10 % SDS polyacylamide gel)
Sample
Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
Specification
adipose tissue derived mesenchymal stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Seunghoon Lee

Verified customer

提交于 Apr 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Mouse Tissue sections (mouse skin)
Specification
mouse skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Unfortunately, we do not have information regarding cross reactivity of ab2318 to a PEG2 metabolite like 13,14-dihydro-15-keto PGE2.

DISCOUNT CODE: XXXXXXX
Expiration date: 20-05-2013

I am very pleased to hear you would like to accept our offer and test ab2318 in IP. This code will give you 100% off your next order before the expiration date. To redeem this offer, pleas...

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Thank you for contacting us and your interest in our products.

We unfortunately do not have the Anti-Prostaglandin E2 antibody (ab2318) available without sodium azide present.

We do however have purification kits available from our ...

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Thank you for your inquiry.

I have to confirm thatab2318 is not eligible for the testing programmewith different species.

The target Prostaglandin E2 is not a protein but a lipid. This chemical will be the same independently of the ...

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Thank you for contacting us.


I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a di...

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DISCOUNT CODE: XXXXX Expiration date: 12/05/2012 I am very pleased to hear you would like to accept our offer and testab2318 in Rat in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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