Anti-Prostaglandin E Receptor EP4抗体(ab93486)

概述

  • 产品名称Anti-Prostaglandin E Receptor EP4抗体
    参阅全部 Prostaglandin E Receptor EP4 一抗
  • 描述
    兔多克隆抗体to Prostaglandin E Receptor EP4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human
  • 免疫原

    Synthetic peptide derived from Human Prostaglandin E Receptor EP4

  • 阳性对照
    • IM-9 and SGC cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab93486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/4000. Detects a band of approximately 70 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
  • 组织特异性High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EP 4 antibody
    • EP4 antibody
    • EP4R antibody
    • MGC126583 antibody
    • PE2R4_HUMAN antibody
    • PGE receptor EP4 subtype antibody
    • PGE2 receptor EP4 subtype antibody
    • Prostaglandin E receptor 4 antibody
    • Prostaglandin E receptor 4 subtype EP4 antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin E2 receptor EP4 subtype antibody
    • Prostanoid EP4 receptor antibody
    • PTGER 2 antibody
    • PTGER 4 antibody
    • PTGER2 antibody
    • PTGER4 antibody
    see all

Anti-Prostaglandin E Receptor EP4 antibody 图像

  • All lanes : Anti-Prostaglandin E Receptor EP4 antibody (ab93486) at 1/2000 dilution

    Lane 1 : IM-9 cell lysate at 43 µg
    Lane 2 : IM-9 cell lysate at 53 µg
    Lane 3 : SGC cell lysate at 43 µg
    Lane 4 : SGC cell lysate at 53 µg


    Predicted band size : 53 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab93486 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93486, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Prostaglandin E Receptor EP4 antibody (ab93486)参考文献

This product has been referenced in:
  • Torres-Atencio I  et al. Prostaglandin E2 prevents hyperosmolar-induced human mast cell activation through prostanoid receptors EP2 and EP4. PLoS One 9:e110870 (2014). Read more (PubMed: 25329458) »
  • Srivastava T  et al. Cyclooxygenase-2, prostaglandin E2, and prostanoid receptor EP2 in fluid flow shear stress-mediated injury in the solitary kidney. Am J Physiol Renal Physiol 307:F1323-33 (2014). Read more (PubMed: 25234310) »

See all 2 Publications for this product

Product Wall

For a Jurkat cell lysate for western blotting, I recommend item ab7899:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=7899).

The lysate is presented in SDS-PAGE loading buffer:

50 mM Tris-H...

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Thank you for contacting us. Your customer can use a Jurkat cell lysate as a positive control. We have the lysate as ab7899: Click here (or use the following: http://www.abcam.com/index.html?datasheet=7899). The Jurkat cell lysate is also an appropriat...

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