Anti-Prostaglandin E Receptor EP4抗体(ab45295)

概述

  • 产品名称Anti-Prostaglandin E Receptor EP4抗体
    参阅全部 Prostaglandin E Receptor EP4 一抗
  • 描述
    兔多克隆抗体to Prostaglandin E Receptor EP4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Prostaglandin E Receptor EP4.

    (Peptide available as ab45294.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Raji and Ramos

性能

应用

Our Abpromise guarantee covers the use of ab45295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
  • 组织特异性High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EP 4 antibody
    • EP4 antibody
    • EP4R antibody
    • MGC126583 antibody
    • PE2R4_HUMAN antibody
    • PGE receptor EP4 subtype antibody
    • PGE2 receptor EP4 subtype antibody
    • Prostaglandin E receptor 4 antibody
    • Prostaglandin E receptor 4 subtype EP4 antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin E2 receptor EP4 subtype antibody
    • Prostanoid EP4 receptor antibody
    • PTGER 2 antibody
    • PTGER 4 antibody
    • PTGER2 antibody
    • PTGER4 antibody
    see all

Anti-Prostaglandin E Receptor EP4 antibody 图像

  • ICC/IF image of ab45295 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45295, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (5 min) HeLa cells at 5µg/ml.
  • Anti-Prostaglandin E Receptor EP4 antibody (ab45295) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Prostaglandin E Receptor EP4 antibody (ab45295)参考文献

ab45295 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

You would probably be best suited using a kit that is able to isolate the membrane fraction completely if you are looking at a membrane protein like Prostaglandin E Receptor EP4. You may want to use a kit like our ab...

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Thank you for this feedback! I am happy to hear that it now works :-)

By the way, if you like you could submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this vial of antibody.

The details you have kindly provided will provide us with vital...

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For a Jurkat cell lysate for western blotting, I recommend item ab7899:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=7899).

The lysate is presented in SDS-PAGE loading buffer:

50 mM Tris-H...

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Thank you for contacting us. Your customer can use a Jurkat cell lysate as a positive control. We have the lysate as ab7899: Click here (or use the following: http://www.abcam.com/index.html?datasheet=7899). The Jurkat cell lysate is also an appropriat...

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