Anti-Prostaglandin D2 Receptor抗体(ab99446)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-Prostaglandin D2 Receptor抗体
参阅全部 Prostaglandin D2 Receptor 一抗 -
描述兔多克隆抗体to Prostaglandin D2 Receptor
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4; Raw 264.7; A549; HepG2 and in the following tissue lysates: Mouse lung; Mouse brain; Mouse kidney; Rat small intestine; Rat kidney.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab99446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
靶标
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功能Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate.
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组织特异性Expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes.
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疾病相关Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.
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序列相似性Belongs to the G-protein coupled receptor 1 family.
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细胞定位Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 5729 Human
- Entrez Gene: 19214 Mouse
- Entrez Gene: 498475 Rat
- Omim: 604687 Human
- SwissProt: Q13258 Human
- SwissProt: P70263 Mouse
- SwissProt: Q9R261 Rat
- Unigene: 306831 Human
see all -
别名
- AS 1 antibody
- AS1 antibody
- ASRT 1 antibody
see all
图片
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Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 :EL4 whole cell lysate (ab7183)
Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Small Intestine (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
文献
This product has been referenced in:
- Guan NN et al. Prostaglandin D2 effects and DP1 /DP2 receptor distribution in guinea pig urinary bladder out-flow region. J Cell Mol Med 21:234-243 (2017). IHC . Read more (PubMed: 27664012) »
客户评价及客户问答
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