Anti-Prostaglandin D2 Receptor抗体(ab99446)

概述

  • 产品名称Anti-Prostaglandin D2 Receptor抗体
    参阅全部 Prostaglandin D2 Receptor 一抗
  • 描述
    兔多克隆抗体to Prostaglandin D2 Receptor
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Mouse Prostaglandin D2 Receptor aa 300 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P70263
    (Peptide available as ab111326)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4; Raw 264.7; A549; HepG2 and in the following tissue lysates: Mouse lung; Mouse brain; Mouse kidney; Rat small intestine; Rat kidney.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate.
  • 组织特异性Expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes.
  • 疾病相关Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AS 1 antibody
    • AS1 antibody
    • ASRT 1 antibody
    • ASRT1 antibody
    • DP antibody
    • MGC49004 antibody
    • OTTHUMP00000178986 antibody
    • PD2R_HUMAN antibody
    • PGD receptor antibody
    • PGD2 receptor antibody
    • Prostaglandin D2 receptor (DP) antibody
    • Prostaglandin D2 receptor antibody
    • Prostanoid DP receptor antibody
    • Ptgdr antibody
    see all

Anti-Prostaglandin D2 Receptor antibody 图像

  • All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : EL4 whole cell lysate (ab7183)
    Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Small Intestine (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-Prostaglandin D2 Receptor antibody (ab99446)参考文献

ab99446 has not yet been referenced specifically in any publications.

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