Anti-Prostaglandin D2 Receptor抗体(ab99446)
Key features and details
- Rabbit polyclonal to Prostaglandin D2 Receptor
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Prostaglandin D2 Receptor抗体
参阅全部 Prostaglandin D2 Receptor 一抗 -
描述
兔多克隆抗体to Prostaglandin D2 Receptor -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4; Raw 264.7; A549; HepG2 and in the following tissue lysates: Mouse lung; Mouse brain; Mouse kidney; Rat small intestine; Rat kidney.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99446于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
靶标
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功能
Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate. -
组织特异性
Expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes. -
疾病相关
Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5729 Human
- Entrez Gene: 19214 Mouse
- Entrez Gene: 498475 Rat
- Omim: 604687 Human
- SwissProt: Q13258 Human
- SwissProt: P70263 Mouse
- SwissProt: Q9R261 Rat
- Unigene: 306831 Human
see all -
别名
- AS 1 antibody
- AS1 antibody
- ASRT 1 antibody
see all
图片
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All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 :EL4 whole cell lysate (ab7183)
Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Small Intestine (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab99446 被引用在 3 文献中.
- Sun LQ et al. Effects of MicroRNA-592-5p on Hippocampal Neuron Injury Following Hypoxic-Ischemic Brain Damage in Neonatal Mice - Involvement of PGD2/DP and PTGDR. Cell Physiol Biochem 45:458-473 (2018). PubMed: 29402808
- Guan NN et al. Prostaglandin D2 effects and DP1 /DP2 receptor distribution in guinea pig urinary bladder out-flow region. J Cell Mol Med 21:234-243 (2017). IHC . PubMed: 27664012
- Guan NN et al. Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2. Br J Pharmacol 172:4024-37 (2015). PubMed: 25917171