概述

  • 产品名称Anti-ProSAAS抗体
    参阅全部 ProSAAS 一抗
  • 描述
    小鼠单克隆抗体to ProSAAS
  • 经测试应用适用于: WB, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 173-261 of Human PCSK1N with proprietary 26 kDa tag (NP_037403).

  • 阳性对照
    • PCSK1N transfected 293T cell line lysate; Human cerebellum tissue

性能

应用

Our Abpromise guarantee covers the use of ab118352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

靶标

  • 功能May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity. The function of the processed secreted peptides is not known.
  • 组织特异性Expressed in brain and pancreas.
  • 结构域ProSAAS(1-180) increases secretion of enzymatically inactive PCSK1.
    The C-terminal inhibitory domain is involved in inhibtion of PCSK1. It corresponds to the probable processing intermediate Big PEN-LEN, binds to PCSK1 in vitro and contains the hexapeptide L-L-R-V-K-R, which, as a synthetic peptide, is sufficient for PCSK1 inhibition.
  • 翻译后修饰Proteolytically cleaved in the Golgi.
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Secreted. Golgi apparatus > trans-Golgi network. A N-terminal processed peptide, probably Big SAAS or Little SAAS, is accumulated in cytoplasmic protein tau deposits in frontotemporal dementia and parkinsonism linked to chromosome 17 (Pick disease), Alzheimer disease and amyotrophic lateral sclerosis-parkinsonism/dementia complex 1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • b-LEN antibody
    • b-PEN-LEN antibody
    • b-SAAS antibody
    • Big LEN antibody
    • granin like neuroendocrine peptide antibody
    • l-LEN antibody
    • l-SAAS antibody
    • N-proSAAS antibody
    • OTTHUMP00000032426 antibody
    • PCSK1_HUMAN antibody
    • Pcsk1n antibody
    • pro-SAAS antibody
    • Proprotein convertase 1 inhibitor antibody
    • Proprotein convertase subtilisin/kexin type 1 inhibitor antibody
    • PROSAAS antibody
    • SAAS antibody
    • SAAS CT(1-49) antibody
    • SAAS CT(25-40) antibody
    see all

Anti-ProSAAS antibody 图像

  • Anti-ProSAAS antibody (ab118352) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 27 kDa
  • All lanes : Anti-ProSAAS antibody (ab118352) at 5 µg/ml

    Lane 1 : PCSK1N transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 27 kDa
  • ab118352, at 3 µg/ml staining PCSK1N in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • Detection limit for ab118352 is 0.1 ng/ml as a capture antibody.

Anti-ProSAAS antibody (ab118352)参考文献

ab118352 has not yet been referenced specifically in any publications.

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