概述

  • 产品名称Anti-Proprotein Convertase 2抗体
    参阅全部 Proprotein Convertase 2 一抗
  • 描述
    兔多克隆抗体to Proprotein Convertase 2
  • 经测试应用适用于: ICC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Proprotein Convertase 2 aa 622-638.
    Sequence:

    ELDEAVERSLQSILRKN


    (Peptide available as ab5012)

  • 阳性对照
    • WB: TC 6 cell extract.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab3533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/20 - 1/200.
ICC/IF 1/20 - 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Proprotein Convertase 2 peptide (ab5012).

This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.

靶标

Anti-Proprotein Convertase 2 antibody 图像

  • Anti-Proprotein Convertase 2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg

    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescent analysis of Proprotein Convertase 2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of Proprotein Convertase 2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-Proprotein Convertase 2 antibody (ab3533)参考文献

This product has been referenced in:
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). Read more (PubMed: 18706454) »

See 1 Publication for this product

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