Anti-Prolyl Endopeptidase抗体(ab58988)

概述

  • 产品名称Anti-Prolyl Endopeptidase抗体
    参阅全部 Prolyl Endopeptidase 一抗
  • 描述
    兔多克隆抗体to Prolyl Endopeptidase
  • 特异性ab58988 does not recognize other Prolyl Endopeptidase family members.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide based on the amino terminal end of human Prolyl Endopeptidase. (Peptide available as ab63973.) Immunizing peptide available on request, please contact a customer service representative to ask for availability.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-Fr
  • 应用说明WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 81 kDa.
    A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.

    EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Prolyl Endopeptidase antibody 图像

    • All lanes : Anti-Prolyl Endopeptidase antibody (ab58988) at 1/1000 dilution

      Lane 1 : Madin Darby canine kidney (MDCK) cell lysate at 10 µl
      Lane 2 : Madin Darby canine kidney (MDCK) cell lysate at 5 µl


      Predicted band size : 81 kDa

    Anti-Prolyl Endopeptidase antibody (ab58988)参考文献

    This product has been referenced in:
    • Justa-Schuch D  et al. The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus. Cell Mol Life Sci 71:3611-26 (2014). WB . Read more (PubMed: 24562348) »
    • Kim JD  et al. Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice. Proc Natl Acad Sci U S A 111:11876-81 (2014). Read more (PubMed: 25071172) »

    See all 5 Publications for this product

    Product Wall

    Thank you for contacting us.

    Based on a 95% homology between the bovine and human forms of the protein, each of the three antibodies should cross react with bovine prolyl endopeptidase. As none of these product has been tested in bovine howe...

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    Thank you for contacting Abcam. The immunogen sequence for ab58988 is considered to be proprietary and so I am unable to access the information that you require. I have looked through all of the other Prolyl Endopeptidase antibodies in our catalogue an...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
    Username

    Mrs. Lisa Bruce

    Verified customer

    提交于 Sep 26 2011

    Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"