Anti-Prolyl Endopeptidase抗体(ab110857)

概述

  • 产品名称
    Anti-Prolyl Endopeptidase抗体
    参阅全部 Prolyl Endopeptidase 一抗
  • 描述
    山羊多克隆抗体to Prolyl Endopeptidase
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QYIVGRSRKQSNP

    , corresponding to internal sequence amino acids 657-669 of Human Prolyl Endopeptidase.

  • 阳性对照
    • Human kidney and tonsil tissues.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

图片

  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Bruce LA  et al. Neuropeptidase activity is down-regulated by estradiol in steroid-sensitive regions of the hypothalamus in female mice. Neuropeptides 46:167-72 (2012). Read more (PubMed: 22672888) »

See 1 Publication for this product

客户评价及客户问答

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Oct 23 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Rat Tissue sections (kidney)
Specification
kidney
Permeabilization
Yes - TritonX-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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