Anti-Prolyl Endopeptidase抗体(ab110857)

概述

  • 产品名称Anti-Prolyl Endopeptidase抗体
    参阅全部 Prolyl Endopeptidase 一抗
  • 描述
    山羊多克隆抗体to Prolyl Endopeptidase
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QYIVGRSRKQSNP

    , corresponding to internal sequence amino acids 657-669 of Human Prolyl Endopeptidase.

  • 阳性对照
    • Human kidney and tonsil tissues.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab110857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

Anti-Prolyl Endopeptidase antibody 图像

  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

Anti-Prolyl Endopeptidase antibody (ab110857)参考文献

ab110857 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
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提交于 Oct 23 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Rat Tissue sections (kidney)
Specification kidney
Permeabilization Yes - TritonX-100
Fixative Formaldehyde
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提交于 Mar 19 2014

Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"