存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
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纯度 Immunogen affinity purified
纯化说明 ab86094 was affinity purified using an epitope specific to Proliferation Associated Nucleolar Antigen immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
应用说明 Is unsuitable for IP.
相关性 Proliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis.
NUCLEOLAR ANTIGEN p120 antibody
Anti-Proliferation Associated Nucleolar Antigen antibody 图像
Western blot - Proliferation Associated Nucleolar Antigen antibody (ab86094)
All lanes : Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg/ml Lane 2 : HeLa whole cell lysate at 15 µg/ml Lane 3 : HeLa whole cell lysate at 5 µg/ml Predicted band size : 95 kDa Observed band size : 117 kDa ( why is the actual band size different from the predicted?) Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands. Exposure time : 60 seconds
Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"