Anti-Proinsulin抗体[3A1] (ab8301)

概述

  • 产品名称
    Anti-Proinsulin抗体[3A1]
  • 描述
    小鼠单克隆抗体[3A1] to Proinsulin
  • 特异性
    Kd for this antibody is >1.1 x 10-8M This antibody is specific for proinsulin. It does not recognise insulin or C-peptide.
  • 经测试应用
    适用于: ICC/IF, ELISA, IHC-Fr, RIAmore details
  • 种属反应性
    与反应: Human
    不与反应: Cow, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • 表位
    This antibody reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.

性能

应用

Our Abpromise guarantee covers the use of ab8301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

靶标

  • 相关性
    A precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • INS antibody
    • insulin antibody
    • IRDN antibody
    • Proinsulin precursor antibody

Anti-Proinsulin antibody [3A1] 图像

  • ab8301 at a 1/500 dilution staining human pancreas tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 1 hour and then detected using an HRP conjugated system.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

    See Abreview

Anti-Proinsulin antibody [3A1] (ab8301)参考文献

This product has been referenced in:
  • Matsunaga K  et al. Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2. J Cell Sci 130:541-550 (2017). Read more (PubMed: 27927751) »
  • Cao M  et al. PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol 11:e1001541 (2013). Read more (PubMed: 23630453) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - citrate buffer
Specification
Brain
Blocking step
10% Methanol+3% H2O2 in PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 23 2015

Vielen Dank für Ihre Anfrage. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 auch nicht mit Mausproben im ELISA funktioniert. Wenn ich mich richtig erinnere, hatten wir am Telefon jedoch auch besprochen, dass ab8301 bisher nicht in der Mau...

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Vielen Dank für diese Rückmeldung. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 nicht optimal für Ihre Zwecke geeignet ist. Leider ist eine nur sehr geringe Verdünnung keiner der Gründe, bei dem es mir erlaubt ist, einen kostenlosen Ersa...

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Thank you for your inquiry. Ab8301 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. According to our info, ab8301 has been tested only with unprocessed proinsulin. I hope that this information is helpfu...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (frozen and FFPE pancreas)
Specification
frozen and FFPE pancreas
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

Abcam user community

Verified customer

提交于 Dec 12 2005

Clone 3A1 (ab8301) reacts with the site of connection of C-peptide and C-end of B-chain of the proinsulin molecule. Clones 1D6 (ab8299) and 1D3 (ab8300) react with different epitopes on the human proinsulin molecule, both of each are generated by the ...

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These are some of our newest products and all the information we have on each of them is featured on the datasheets. As yet, there are no published references. Pricing information in GBP, EUR and USD can be found by clicking on the "prices and buying" ...

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The antibody has not yet been tested on paraffin embedded sections, although it has been shown to be suitable for use in IHC with frozen sections. It has not been tested on mouse although we note that it does not cross-react with bovine or porcine proi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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