概述

  • 产品名称
    Anti-Prohibitin抗体
    参阅全部 Prohibitin 一抗
  • 描述
    兔多克隆抗体to Prohibitin
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to near C terminus of human Prohibitin protein.

  • 阳性对照
    • Human heart.

性能

应用

Our Abpromise guarantee covers the use of ab70672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
    • 组织特异性
      Widely expressed in different tissues.
    • 序列相似性
      Belongs to the prohibitin family.
    • 发展阶段
      Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
    • 细胞定位
      Mitochondrion inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Epididymis luminal protein 215 antibody
      • Epididymis secretory sperm binding protein Li 54e antibody
      • HEL 215 antibody
      • HEL S 54e antibody
      • PHB antibody
      • PHB_HUMAN antibody
      • PHB1 antibody
      • Prohibitin antibody
      see all

    图片

    • ab70672, at 1/100 dilution, staining Prohibitin in formalin fixed, paraffin embedded human heart tissue by immunohistochemistry.

    文献

    This product has been referenced in:
    • Zhu X  et al. Long-circulating siRNA nanoparticles for validating Prohibitin1-targeted non-small cell lung cancer treatment. Proc Natl Acad Sci U S A 112:7779-84 (2015). Read more (PubMed: 26056316) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you very much for your patience.

    I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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