概述

  • 产品名称Anti-Prohibitin抗体
    参阅全部 Prohibitin 一抗
  • 描述
    小鼠单克隆抗体to Prohibitin
  • 经测试应用适用于: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-273 of Human Prohibitin

性能

应用

Our Abpromise guarantee covers the use of ab55618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • 组织特异性Widely expressed in different tissues.
  • 序列相似性Belongs to the prohibitin family.
  • 发展阶段Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Anti-Prohibitin antibody 图像

  • ICC/IF image of rat primary mixed glial culture stained with ab55618. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab55618, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Prohibitin antibody (ab55618) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • Prohibitin antibody (ab55618) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.


  • Predicted band size : 30 kDa
    Prohibitin antibody (ab55618) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab55618 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55618, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Prohibitin antibody (ab55618)参考文献

This product has been referenced in:
  • Kamal AH  et al. Investigation of adipocyte proteome during the differentiation of brown preadipocytes. J Proteomics 94:327-36 (2013). Read more (PubMed: 24129212) »
  • Perciavalle RM  et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). Read more (PubMed: 22544066) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Mature spermatid/Adult testes)
Permeabilization Yes - 0.4% PBS Triton
Specification Mature spermatid/Adult testes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 11 2015

Application Western blot
Sample Mouse Tissue lysate - other (Liver Mitochondrial fraction)
Loading amount 20 µg
Specification Liver Mitochondrial fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mixed Glial culture)
Specification Mixed Glial culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.1x PBS plus 0.3xTriton x
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 2 µg
Specification HL60
Gel Running Conditions Reduced Denaturing (17%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 16 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - Triton 0.6%
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 23°C
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提交于 Dec 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"