The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
Use a concentration of 1 - 10 µg/ml.
Use a concentration of 5 µg/ml.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
组织特异性Widely expressed in different tissues.
序列相似性Belongs to the prohibitin family.
发展阶段Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
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Anti-Prohibitin antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody (ab55618)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of rat primary mixed glial culture stained with ab55618. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab55618, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HepG2 cells stained with ab55618 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55618, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Prohibitin antibody (ab55618)参考文献
This product has been referenced in:
Kamal AH et al. Investigation of adipocyte proteome during the differentiation of brown preadipocytes. J Proteomics94:327-36 (2013).
Read more (PubMed: 24129212) »
Perciavalle RM et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol14:575-83 (2012).
Read more (PubMed: 22544066) »