Anti-Progesterone Receptor (phospho S190)抗体(ab47751)


  • 产品名称Anti-Progesterone Receptor (phospho S190)抗体
    参阅全部 Progesterone Receptor 一抗
  • 描述
    兔多克隆抗体to Progesterone Receptor (phospho S190)
  • 特异性This antibody is specific for Progesterone Receptor only when phosphorylated at Serine 190.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthesized phosphopeptide derived from human Progesterone Receptor around the phosphorylation site of Serine 190 (G-L-SP-P-A)

  • 阳性对照
    • WB: Extracts from COS7 cells IHC-P: breast carcinoma tissue



Our Abpromise guarantee covers the use of ab47751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • 功能The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 细胞定位Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Anti-Progesterone Receptor (phospho S190) antibody 图像

  • All lanes : Anti-Progesterone Receptor (phospho S190) antibody (ab47751) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, untreated
    Lane 2 : Extracts from COS7 cells, treated with EGF

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
  • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab47751 at a 1/50 dilution and Alkaline Phosphatase Goat Anti-rabbit IgG (H+L) secondary antibody.

Anti-Progesterone Receptor (phospho S190) antibody (ab47751)参考文献

ab47751 has not yet been referenced specifically in any publications.

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