Anti-Progesterone Receptor抗体[YR85] (ab32085)




Our Abpromise guarantee covers the use of ab32085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/250.
IP 1/150.
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻译后修饰Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • 细胞定位Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Information by UniProt
    • 数据库链接
    • 别名
      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    Anti-Progesterone Receptor antibody [YR85] 图像

    • ICC/IF image of ab32085 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32085 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-Progesterone Receptor antibody [YR85] (ab32085) at 1/10000 dilution + T47D cell lysate

      Predicted band size : 99 kDa
      Observed band size : 99,135 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of progesterone receptor expression in paraffin embedded human breast carcinoma, using 1/100 ab32085.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Progesterone Receptor antibody [YR85] (ab32085)参考文献

    This product has been referenced in:
    • Yin K Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade. Onco Targets Ther 8:1069-74 (2015). IHC-P ; Human . Read more (PubMed: 25999742) »
    • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 23911795) »

    See all 8 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Mammary epithelial cells)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
    Permeabilization No
    Specification Mammary epithelial cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde

    Mrs. Katie Elliott

    Verified customer

    提交于 Jul 15 2016

    Es tut mir leid, dass der Antikörper nicht so funktioniert hat wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, Gutschriften für Sie auszustellen. Sie können die Gutschriften auf folgende Weise verwenden:

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    Wir testen unsere Chargen immer, bevor wir unser Datenblatt aktualisieren, und uns ist bei diesem Antikörper aufgefallen, dass die Spezies- Reaktivität in Maus und Ratte eben nicht konstant, bzw gar nicht mehr gegeben war, so dass wir Maus und Ratte al...

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    Vielen Dank für Ihre Email.

    Wir haben das Datenblatt im März 2011 geändert, allerdings habe ich keine Hinweise darauf gefunden, dass wir eine Kampagne gestartet haben. Ich habe dies jetzt nachgeholt.

    Wenn Sie mir bitte mitteilen, ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (human breast cancer xenograft (MCF7))
    Specification human breast cancer xenograft (MCF7)
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
    Permeabilization No
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 Apr 26 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (breast cancer)
    Specification breast cancer
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate - 20 minutes 100oC
    Permeabilization No

    Abcam user community

    Verified customer

    提交于 Feb 09 2009

    Application ChIP
    Sample Human Cell lysate - whole cell (Uterus)
    Specification Uterus
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step Semiquantitative PCR
    Positive control RNA Polymerase II
    Negative control Rabbit IgG

    Abcam user community

    Verified customer

    提交于 Oct 17 2008

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab32085 in immunohistochemistry. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and...

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