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Recombinant fragment corresponding to Progesterone Receptor.
Our Abpromise guarantee covers the use of ab16661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100. PubMed: 18617611|
|WB||1/200. Predicted molecular weight: 99,120 kDa.|
|IHC-P||1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.
ab16661 staining rat ovary tissue sections by IHC-P. Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C. A HRP conjugated goat anti-rabbit was used as the secondary antibody.
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