Anti-Progesterone Receptor抗体(ab97801)

概述

  • 产品名称Anti-Progesterone Receptor抗体
    参阅全部 Progesterone Receptor 一抗
  • 描述
    兔多克隆抗体to Progesterone Receptor
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 18-82 (PPSPEVGSPL LCRPAAGPFP GSQTSDTLPE VSAIPISLDG LLFPRPCQGQ DPSDEKTQDQ QSLSD) of Human Progesterone Receptor (NP_000917)

  • 阳性对照
    • H1299 or MOLT4 lysates A549 xenograft (IHC) A549 cells (IF)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab97801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 细胞定位Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Anti-Progesterone Receptor antibody 图像

  • Anti-Progesterone Receptor antibody (ab97801) at 1/500 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97801 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97801 at 1/100 dilution.

Anti-Progesterone Receptor antibody (ab97801)参考文献

ab97801 has not yet been referenced specifically in any publications.

Product Wall

We have several polyclonal anti-progesterone receptor antibodies, but none that we know are specific for isoform A. For isoform B, we assume that any antibody that is raised against the amino acid sequence that isoform A is missing, (amino acids 1-164,...

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Thank you for sending the image of the western blot. The receptor is expected to run at about 100kDa, which I assume is the size of the most intense band on the blot. The higher molecular weight band that you identify as being >170kDa could be a dimer,...

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Thank you for sending the details of your protocol. I am sorry to read that you are seeing a an unexpected band (or bands). Can you please describe where you are seeing bands on your blot? If you can attach an image with your reply, that will help us a...

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