Anti-Progesterone Receptor抗体(ab90577)


  • 产品名称Anti-Progesterone Receptor抗体
    参阅全部 Progesterone Receptor 一抗
  • 描述
    小鼠单克隆抗体to Progesterone Receptor
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to N terminal amino acids 1-111 of Human Progesterone Receptor (NP_000917) with a proprietary tag.

  • 阳性对照
    • WB: A431 cell lysate. IHC: human endometrium tissue ICC/IF: A431 cells.




Our Abpromise guarantee covers the use of ab90577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 1.5 µg/ml. Antigen retrieval is not essential but may optimise staining. Recommended method: place sample in 1X citrate buffer pH 6.0 and microwave at 750W for 20 minutes.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 99 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻译后修饰Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • 细胞定位Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Information by UniProt
    • 数据库链接
    • 别名
      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    Anti-Progesterone Receptor antibody 图像

    • Anti-Progesterone Receptor antibody (ab90577) at 5 µg/ml + A431 cell lysate at 50 µg

      Predicted band size : 99 kDa
    • ab90577 at 1.5 µg/ml staining Progesterone Receptor in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry,
    • ab90577 at 10µg/ml staining Progesterone Receptor in A431 cells by Immunofluorescence.

    Anti-Progesterone Receptor antibody (ab90577)参考文献

    ab90577 has not yet been referenced specifically in any publications.

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