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Synthetic non-phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of serine 294 (G-R-SP-P-L).
Our Abpromise guarantee covers the use of ab63605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
Paraffin-embedded human liver carcinoma tissue stained for Progesterone Receptor with ab63605 at 1/50 dilution in immunohistochemical analysis.
In the right-hand panel the sample is incubated with the immunizing peptide.
ab63605 staining Progesterone Receptor in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris_EDTA. Samples were incubated with primary antibody (1/100) for 10 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.