Anti-Profilin 1抗体- Carboxyterminal end (ab50667)
Key features and details
- Rabbit polyclonal to Profilin 1 - Carboxyterminal end
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Profilin 1抗体- Carboxyterminal end
参阅全部 Profilin 1 一抗 -
描述
兔多克隆抗体to Profilin 1 - Carboxyterminal end -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide:
CYEMASHLRRSQY
conjugated to KLH, corresponding to C terminal amino acids 128-140 of Human Profilin 1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50667于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
说明 |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
序列相似性
Belongs to the profilin family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 18643 Mouse
- SwissProt: P62962 Mouse
- Unigene: 2647 Mouse
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别名
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
图片
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Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.
Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.
Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab50667 被引用在 13 文献中.
- Drazic A et al. The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1. J Mol Biol 434:167397 (2022). PubMed: 34896361
- Zi J et al. PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK. Cells 11:N/A (2022). PubMed: 36291059
- Nejedlá M et al. The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 4:N/A (2021). PubMed: 33184056
- Nekouei M et al. Mutant Profilin1 Aggregation in Amyotrophic Lateral Sclerosis: An in Vivo Biochemical Analysis. Basic Clin Neurosci 12:213-222 (2021). PubMed: 34925718
- Rebowski G et al. Mechanism of actin N-terminal acetylation. Sci Adv 6:eaay8793 (2020). PubMed: 32284999