概述

  • 产品名称Anti-Profilin 1抗体
    参阅全部 Profilin 1 一抗
  • 描述
    兔多克隆抗体to Profilin 1
  • 特异性Sequence similarity is 70% similar to the corresponding sequence in Profilin 2.
  • 经测试应用适用于: Indirect ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Synthetic peptide:

    AGWNAYIDNLMADGTC

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Profilin 1

  • 阳性对照
    • Whole extracts of mouse NIH-3T3 cells, human HeLa cells (if applying chemiluminescent detection reagent) and rat NRK cells (via indirect immunofluorescence).

性能

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应用

Our Abpromise guarantee covers the use of ab50668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use a concentration of 10 - 20 µg/ml. (recommended using rat NRK cells).
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). (using extracts of mouse NIH-3T3 and human HeLa cells, applying a chemiluminescent detection reagent).

靶标

Anti-Profilin 1 antibody 图像

  • All lanes : Anti-Profilin 1 antibody (ab50668) at 0.5 µg/ml

    Lane 1 : Whole Mouse 3T3 cell extract
    Lane 2 : Whole Mouse 3T3 cell extract with Profilin 1 (N-terminal) immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Predicted band size : 15 kDa
    Observed band size : 15 kDa

Anti-Profilin 1 antibody (ab50668)参考文献

ab50668 has not yet been referenced specifically in any publications.

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