The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 - 20 µg/ml. (recommended using rat NRK cells).
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). (using extracts of mouse NIH-3T3 and human HeLa cells, applying a chemiluminescent detection reagent).
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.