Anti-ProDynorphin抗体(ab11137)

概述

  • 产品名称
    Anti-ProDynorphin抗体
    参阅全部 ProDynorphin 一抗
  • 描述
    兔多克隆抗体to ProDynorphin
  • 特异性
    This antibody blocks specifically with leumorphin (rat only). Cells are found in the paraventricular and supraoptic nucleus and fibres in the lateral hypothalamus in rat brain of colchicine treated 4% FA perfused tissue.
  • 经测试应用
    适用于: IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    RSQENPNTYSEDLDV

    (Rat)

  • 常规说明
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
    Whole serum is supplied - diluted.
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200 - 1/2000.

靶标

  • 功能
    Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
    Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
    Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
  • 疾病相关
    Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.
  • 序列相似性
    Belongs to the opioid neuropeptide precursor family.
  • 翻译后修饰
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCA antibody
    • Alpha neoendorphin antibody
    • Beta neoendorphin antibody
    • Beta-neoendorphin-dynorphin antibody
    • Big Dyn antibody
    • Big dynorphin antibody
    • Dyn antibody
    • Dyn-A17 antibody
    • Dyn-B antibody
    • Dynorphin A antibody
    • Dynorphin A(1-13) antibody
    • Dynorphin A(1-17) antibody
    • Dynorphin A(1-8) antibody
    • Dynorphin B antibody
    • Dynorphin B(1-13) antibody
    • Dynorphin B-29 antibody
    • Enkephalin B antibody
    • Leu enkephalin antibody
    • Leumorphin antibody
    • Neoendorphin dynorphin enkephalin prepropeptide antibody
    • PDYN antibody
    • PDYN_HUMAN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Preproenkephalin B antibody
    • Prodynorphin antibody
    • Proenkephalin B antibody
    • Rimorphin antibody
    • SCA23 antibody
    see all

图片

  • ab11137 staining ProDynorphin in Rat brain tissue by Immunohistochemistry - Free floating sections. The tissue was fixed by transcardial perfusion with 4% paraformaldehyde, 0.1% glutaraldeyde in 0.1M phosphate buffer (pH 7.4). The brain was postfixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH7.4) overnight at 4°C and then cut into 50 µm-thick parasagittal sections with a vibrating microtome. The primary antibody was diluted 1/100 in and incubated with the sample for 16 hours at 20°C. A Cy3®-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500.

    See Abreview

文献

This product has been referenced in:
  • Whitfield TW  et al. ? Opioid receptors in the nucleus accumbens shell mediate escalation of methamphetamine intake. J Neurosci 35:4296-305 (2015). Read more (PubMed: 25762676) »
  • Romero-Picó A  et al. Hypothalamic ?-opioid receptor modulates the orexigenic effect of ghrelin. Neuropsychopharmacology 38:1296-307 (2013). Read more (PubMed: 23348063) »

See all 3 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formalin
Username

Allisa Song

Verified customer

提交于 Feb 26 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA+NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Cortex)
Specification
Cortex
Permeabilization
Yes - 0.3% Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 18 2014

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Username

Dr. Kouichi Nakamura

Verified customer

提交于 Mar 24 2011

I have contacted our accounting department and you will be issued a refund for your order for ab11137. This process does take a couple of weeks and if you have any questions regarding the refund please email: accounts@abcam.com If you have any future ...

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Thank you for your patience and the details that you have provided us with. To our knowledge, this antibody has only been tested for application in IHC-frozen sections. We do not know how the antibody works in paraformaldehyde or formalin-fixed section...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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