Anti-Procollagen Type 1抗体[M-58] (ab64409)

概述

  • 产品名称
    Anti-Procollagen Type 1抗体[M-58]
  • 描述
    大鼠单克隆抗体[M-58] to Procollagen Type 1
  • 特异性
    ab64409 recognizes Human procollagen I. Labels the amino-terminal pro-peptide. Localized intracellularly in cells producing pro-collagen I. Does not stain mature collagen fibers in tissue. The clone number has been updated from (2Q576) to (M-58) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
    不适用于: WB
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Human procollagen I.

  • 阳性对照
    • Human skin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: Tissue culture supernatant
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    M-58
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend antigen retrieval with 1% trypsin for 20 minutes at room temperature.
IHC-Fr 1/10000 - 1/50000.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 相关性
      Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Procollagen type 1 is mainly synthesized by osteoblasts and, after cleavage of the N- and C-terminal extension peptides, is utilized for collagen fibril deposition in the osteoid tissue.
    • 细胞定位
      Secreted
    • 数据库链接
    • 别名
      • Alpha 1 type I collagen antibody
      • Alpha 2 type I collagen antibody
      • COL1A1 antibody
      • COL1A2 antibody

    图片

    • ab64409 staining Procollagen Type 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.25% serum for 15 minutes at 25°C ; enzymatic antigen retrieval using Proteinase K was performed. Samples were incubated with primary antibody (1/800 in serum free antibody diluent) for 30 minutes at 25°C. A Biotin-conjugated Rabbit anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • ab64409 staining Procollagen Type 1 in Pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and permeabilized with Triton X100. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Ab98387 (A goat anti-rat DyLight®550 (IgG; 1/500)) was used as the secondary antibody.

      See Abreview

    • ab64409 staining Procollagen Type 1 (green) in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody. Red - Immune Marker, Blue - DAPI.

      See Abreview

    文献

    This product has been referenced in:
    • Xia H  et al. Calcium-binding protein S100A4 confers mesenchymal progenitor cell fibrogenicity in idiopathic pulmonary fibrosis. J Clin Invest N/A:N/A (2017). Read more (PubMed: 28530639) »
    • Shiwen X  et al. A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS One 10:e0126015 (2015). IHC-P ; Human . Read more (PubMed: 25955164) »

    See all 5 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    AB Block Dako X0590 as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Thorsten Cramer

    Verified customer

    提交于 Aug 16 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample
    Pig Tissue sections (Skeletal Muscle)
    Specification
    Skeletal Muscle
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 04 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Cardiac Muscle)
    Specification
    Cardiac Muscle
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 30 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Trypsin 1% 20m RT
    Sample
    Human Tissue sections (Skeletal Muscle)
    Specification
    Skeletal Muscle
    Permeabilization
    No
    Fixative
    Methacarn
    Username

    Abcam user community

    Verified customer

    提交于 Oct 18 2013

    DISCOUNT CODE: *
    Expiration date: *

    I am very pleased to hear you would like to accept our offer and test ab66409 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Epithelial)
    Specification
    Epithelial
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - .1% Triton X100
    Username

    Mr. Daniel Holod-Betz

    Verified customer

    提交于 Apr 11 2012

    Thank you for your call today and for keeping me updated about the progress with this antibody.

    As we discussed, I would recommend trying a dilution around 1:600 for aone hourincubation, or 1:800 overnight at 4C. You can also try increasing t...

    Read More

    Thanks for your call today and for letting us know about the trouble with ab64409.

    Please keep me updated about any new results using the antibody as we discussed.

    Let me know if you have any questions or if there is anything else t...

    Read More
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Tracheal Fibroblast)
    Specification
    Tracheal Fibroblast
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 14 2012


    I am very pleased to hear you would like to accept our offer and test ab10559 and ab64409 in samples.This code will give you 1 freeprimary antibodybefore the expiration date for each discount code. To redeem this offer, please submit an Abreview f...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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