概述

  • 产品名称Anti-pro Caspase 9抗体[E84]
    参阅全部 pro Caspase 9 一抗
  • 描述
    兔单克隆抗体[E84] to pro Caspase 9
  • 特异性This antibody only recognises the pro form of Caspase 9 (46kDa)
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near N-terminus of human Caspase-9

  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号E84
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab32068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/25.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
    • 组织特异性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
    • 序列相似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 发展阶段Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
    • 翻译后修饰Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APAF-3 antibody
      • APAF3 antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase-9 subunit p10 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • ICELAP6 antibody
      • MCH6 antibody
      see all

    Anti-pro Caspase 9 antibody [E84] 图像

    • Anti-pro Caspase 9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).

    Anti-pro Caspase 9 antibody [E84] (ab32068)参考文献

    This product has been referenced in:
    • Catuogno S  et al. miR-34c may protect lung cancer cells from paclitaxel-induced apoptosis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22370637) »
    • Hou W  et al. Autophagic degradation of active caspase-8: a crosstalk mechanism between autophagy and apoptosis. Autophagy 6:891-900 (2010). WB . Read more (PubMed: 20724831) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32068.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"