使用敲除细胞株进行验证
重组
RabMAb

Anti-pro Caspase 7抗体[Y33] (ab32067)

概述

  • 产品名称
    Anti-pro Caspase 7抗体[Y33]
  • 描述
    兔单克隆抗体[Y33] to pro Caspase 7
  • 特异性
    The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7.

  • 阳性对照
    • Jurkat cell lysate and human lung squamous carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50.
ICC/IF 1/50.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Overexpression promotes programmed cell death.
    • 组织特异性
      Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
    • 序列相似性
      Belongs to the peptidase C14A family.
    • 翻译后修饰
      Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • apoptosis-related cysteine peptidase antibody
      • Apoptotic protease Mch-3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase-7 subunit p11 antibody
      • CMH-1 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      see all

    Anti-pro Caspase 7 antibody [Y33] 图像



    • Predicted band size : 34 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
      Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 5: HeLa whole cell lysate (20 µg)
      Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

      Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.
    • Anti-pro Caspase 7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    Anti-pro Caspase 7 antibody [Y33] (ab32067)参考文献

    ab32067 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32067.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册