概述

  • 产品名称Anti-pro Caspase 10抗体[E35]
  • 描述
    兔单克隆抗体[E35] to pro Caspase 10
  • 特异性The antibody does not crossreact with other Caspase family members.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to N terminal residues of human Caspase 10 subunit p23/17.

  • 阳性对照
    • WB: Hela cell lysate. IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号E35
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab32155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10.

靶标

  • 相关性Caspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.
  • 数据库链接
  • 别名
    • Apoptotic protease Mch4 antibody
    • CASP10 antibody
    • Caspase 10 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    see all

Anti-pro Caspase 10 antibody [E35] 图像

  • Anti-pro Caspase 10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
  • Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence.
  • Flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative)(green).

Anti-pro Caspase 10 antibody [E35] (ab32155)参考文献

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