Anti-pro Caspase-10抗体[E35] (ab32155)
Key features and details
- Rabbit monoclonal [E35] to pro Caspase-10
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
-
产品名称
Anti-pro Caspase-10抗体[E35]
参阅全部 pro Caspase-10 一抗 -
描述
兔单克隆抗体[E35] to pro Caspase-10 -
宿主
Rabbit -
特异性
The antibody does not crossreact with other Caspase family members. -
经测试应用
适用于: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human pro Caspase-10 aa 200-300 (N terminal). The exact sequence is proprietary.
-
阳性对照
- WB: Hela cell lysate. ICC/IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
克隆
单克隆 -
克隆编号
E35 -
同种型
IgG -
研究领域
相关产品
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
|
|
WB |
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
---|
ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
-
相关性
Caspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis. -
数据库链接
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q8IUP5 Human
- SwissProt: Q92851 Human
-
别名
- Apoptotic protease Mch4 antibody
- CASP10 antibody
- Caspase 10 antibody
see all
图片
-
Anti-pro Caspase-10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence.
-
Intracellular flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative) (green).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab32155 被引用在 4 文献中.
- Wang Y et al. The relationship between liver pathological inflammation degree and pyroptosis in chronic hepatitis B patients. J Med Virol N/A:N/A (2021). PubMed: 34061368
- Han X et al. Zika virus infection induced apoptosis by modulating the recruitment and activation of pro-apoptotic protein Bax. J Virol 95:N/A (2021). PubMed: 33536166
- Lu Z et al. Dracorhodin perchlorate induces apoptosis and G2/M cell cycle arrest in human esophageal squamous cell carcinoma through inhibition of the JAK2/STAT3 and AKT/FOXO3a pathways. Mol Med Rep 20:2091-2100 (2019). PubMed: 31322237
- Coutinho-Camillo CM et al. Caspase expression in oral squamous cell carcinoma. Head Neck 33:1191-8 (2011). IHC ; Human . PubMed: 21755562