Anti-PRMT7抗体(ab59891)

概述

  • 产品名称Anti-PRMT7抗体
    参阅全部 PRMT7 一抗
  • 描述
    兔多克隆抗体to PRMT7
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide in the N terminal region of human PRMT7 conjugated to KLH

  • 阳性对照
    • IHC-P: Human hepatocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500.

靶标

  • 功能Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
  • 疾病相关Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
  • 序列相似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
    Contains 2 SAM-dependent MTase PRMT-type domains.
  • 细胞定位Cytoplasm, cytosol. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
    • ANM7_HUMAN antibody
    • FLJ10640 antibody
    • Histone-arginine N-methyltransferase PRMT7 antibody
    • KIAA1933 antibody
    • Myelin basic protein arginine N methyltransferase antibody
    • OTTHUMP00000174863 antibody
    • PRMT7 antibody
    • Protein arginine methyltransferase 7 antibody
    • Protein arginine N-methyltransferase 7 antibody
    see all

Anti-PRMT7 antibody 图像

  • ab59891, at 1/50 dilution, staining PRMT7 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by Immunohistochemistry. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
  • All lanes : Anti-PRMT7 antibody (ab59891) at 1/500 dilution

    Lane 1 : 293 cell lysate non-transfected
    Lane 2 : 293 cell lysate transiently transfected with the PRMT7 gene

    Lysates/proteins at 2 µg per lane.

Anti-PRMT7 antibody (ab59891)参考文献

ab59891 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (u2os)
Specification u2os
Fixative Formaldehyde
Permeabilization Yes - triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"