概述

  • 产品名称Anti-PRMT7抗体
    参阅全部 PRMT7 一抗
  • 描述
    兔多克隆抗体to PRMT7
  • 特异性ab22110 reacts with protein arginine methyltransferase 7 (PRMT7).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    YSLQRTSPEKNERVR

    , corresponding to internal sequence amino acids 346-360 of Human PRMT7. This amino acid sequence can be found in five different human PRMT7 isoforms: CRA_a (692 amino acids), CRA_b (613 amino acids), CRA_c (465 amino acids), CRA_d (655 amino acids) and CRA_e (567 amino acids)

  • 阳性对照
    • HeLa or NIH 3T3 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab22110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 68 kDa.

靶标

  • 功能Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
  • 疾病相关Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
  • 序列相似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
    Contains 2 SAM-dependent MTase PRMT-type domains.
  • 细胞定位Cytoplasm, cytosol. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
    • ANM7_HUMAN antibody
    • FLJ10640 antibody
    • Histone-arginine N-methyltransferase PRMT7 antibody
    • KIAA1933 antibody
    • Myelin basic protein arginine N methyltransferase antibody
    • OTTHUMP00000174863 antibody
    • PRMT7 antibody
    • Protein arginine methyltransferase 7 antibody
    • Protein arginine N-methyltransferase 7 antibody
    see all

Anti-PRMT7 antibody 图像

  • All lanes : Anti-PRMT7 antibody (ab22110) at 2 µg/ml

    Lane 1 : HeLa Cell Lysate
    Lane 2 : NIH 3T3 Cell Lysate

Anti-PRMT7 antibody (ab22110)参考文献

This product has been referenced in:
  • Verbiest V  et al. Protein arginine (N)-methyl transferase 7 (PRMT7) as a potential target for the sensitization of tumor cells to camptothecins. FEBS Lett 582:1483-9 (2008). WB ; Human . Read more (PubMed: 18381071) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblast cell line)
Loading amount 25 µg
Specification Human fibroblast cell line
Treatment Overexpression of HIS-tagged PRMT-7 expressing plasmid
Gel Running Conditions Reduced Denaturing (10 % SDS-PAGE gel ran in BioRad Tetracell apparatus)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"