概述

  • 产品名称Anti-PRMT6抗体
    参阅全部 PRMT6 一抗
  • 描述
    兔多克隆抗体to PRMT6
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human PRMT6 (C terminal). A region between residue 325 and the C-terminus (residue 375) of PRMT6 (AAK85733.1).
    Database link: 55170

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab72205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
    • 组织特异性Highly expressed in kidney and testis.
    • 序列相似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
      Contains 1 SAM-dependent MTase PRMT-type domain.
    • 翻译后修饰Automethylation enhances its stability and antiretroviral activity.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANM6_HUMAN antibody
      • Chromobox protein homolog 7 antibody
      • FLJ10559 antibody
      • FLJ51477 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
      • Histone-arginine N-methyltransferase PRMT6 antibody
      • HMT1 hnRNP methyltransferase like 6. antibody
      • HRMT1L6 antibody
      • OTTHUMP00000012633 antibody
      • PRMT 6 antibody
      • prmt6 antibody
      • Protein arginine methyltransferase 6 antibody
      • Protein arginine N methyltransferase 6 antibody
      • Protein arginine N-methyltransferase 6 antibody
      see all

    Anti-PRMT6 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRMT6 with ab72205 at 1/5000 (0.2 µg/ml). Detection: DAB.
    • All lanes : Anti-PRMT6 antibody (ab72205) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 42 kDa

    Anti-PRMT6 antibody (ab72205)参考文献

    ab72205 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (u2os)
    Loading amount 80 µg
    Specification u2os
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 10 2011

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (u2os)
    Specification u2os
    Fixative Formaldehyde
    Permeabilization Yes - triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"