• 产品名称Anti-PRMT6抗体
    参阅全部 PRMT6 一抗
  • 描述
    兔多克隆抗体to PRMT6
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PRMT6.

  • 阳性对照
    • This antibody gave a positive signal in Human Testis as well as A498 and HeLa whole cell lysates.



Our Abpromise guarantee covers the use of ab104834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


  • 功能Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
  • 组织特异性Highly expressed in kidney and testis.
  • 序列相似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
    Contains 1 SAM-dependent MTase PRMT-type domain.
  • 翻译后修饰Automethylation enhances its stability and antiretroviral activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANM6_HUMAN antibody
    • Chromobox protein homolog 7 antibody
    • FLJ10559 antibody
    • FLJ51477 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
    • Histone-arginine N-methyltransferase PRMT6 antibody
    • HMT1 hnRNP methyltransferase like 6. antibody
    • HRMT1L6 antibody
    • OTTHUMP00000012633 antibody
    • PRMT 6 antibody
    • prmt6 antibody
    • Protein arginine methyltransferase 6 antibody
    • Protein arginine N methyltransferase 6 antibody
    • Protein arginine N-methyltransferase 6 antibody
    see all

Anti-PRMT6 antibody 图像

  • ab104834 (1/200) staining PRMT6 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • All lanes : Anti-PRMT6 antibody (ab104834) at 1 µg/ml

    Lane 1 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 120 kDa,68 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-PRMT6 antibody (ab104834)参考文献

ab104834 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde

Dr. Kirk McManus

Verified customer

提交于 Sep 06 2013