概述

  • 产品名称Anti-PRMT3抗体- ChIP Grade
    参阅全部 PRMT3 一抗
  • 描述
    兔多克隆抗体to PRMT3 - ChIP Grade
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    STKTHWKQTVFLLEKPF

    , corresponding to C terminal amino acids 475-491 of Human PRMT3

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116

性能

应用

Our Abpromise guarantee covers the use of ab3765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 60 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 10 µg/ml.
ChIP Use 10µl for 106 cells.

靶标

  • 功能Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • 序列相似性Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.
  • 结构域The zinc-finger is responsible for substrate specificity.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2010005E20Rik antibody
    • 2410018A17Rik antibody
    • AL033309 antibody
    • ANM3_HUMAN antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody
    • HMT1 hnRNP methyltransferase like 3 antibody
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae antibody
    • HMT1-like 3 antibody
    • hnRNP methyltransferase like 3 antibody
    • HRMT1L3 antibody
    • PRMT 3 antibody
    • PRMT3 antibody
    • Protein arginine methyltransferase 3 antibody
    • Protein arginine N methyltransferase 3 antibody
    • Protein arginine N-methyltransferase 3 antibody
    see all

Anti-PRMT3 antibody - ChIP Grade 图像

  • ab3765 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3765 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.



  • Predicted band size : 60 kDa
     

     

    Western blot analysis using whole HL60 cell lysate: PRMT3 (Arrow) was detected using ab3765 (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  •  

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3765 to PRMT3 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 µl of ab3765 and 2x106 cells were used in each ChIP experiment.

  • ab3765 staining PRMT3 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PRMT3 antibody - ChIP Grade (ab3765)参考文献

This product has been referenced in:
  • Sabra M  et al. The Tudor protein survival motor neuron (SMN) is a chromatin-binding protein that interacts with methylated lysine 79 of histone H3. J Cell Sci 126:3664-77 (2013). Read more (PubMed: 23750013) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (prostata cancer cell)
Loading amount 10 µg
Specification prostata cancer cell
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jun 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"