Synthetic peptide corresponding to a region within N terminal amino acids 1 - 50 (
) of Human PRMT3 (NP_005779.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7.0-8.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5 - 15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Belongs to the protein arginine N-methyltransferase family.
Contains 1 C2H2-type zinc finger.
The zinc-finger is responsible for substrate specificity.
Information by UniProt
Western blot - Anti-PRMT3 antibody (ab91430)
All lanes : Anti-PRMT3 antibody (ab91430) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 60 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-PRMT3 antibody (ab91430)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91430.
20% of the immunoprecipitate was loaded per lane, and probed with ab91430 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 30 seconds.
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