概述

  • 产品名称Anti-PRMT2抗体- ChIP Grade
    参阅全部 PRMT2 一抗
  • 描述
    兔多克隆抗体to PRMT2 - ChIP Grade
  • 经测试应用适用于: WB, ELISA, IHC-P, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    AIADYAATDETQLSFLR

    , corresponding to amino acids 37-53 of Human PRMT 2.

  • 常规说明

    Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody说明Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.
ChIP Use 10µl for 106 cells.

靶标

  • 功能Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
  • 组织特异性Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
  • 序列相似性Belongs to the protein arginine N-methyltransferase family.
    Contains 1 SH3 domain.
  • 细胞定位Cytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANM2_HUMAN antibody
    • EC 2.1.1. antibody
    • histone arginine N methyltransferase PRMT2 antibody
    • Histone-arginine N-methyltransferase PRMT2 antibody
    • HMT 1 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibody
    • HMT1 antibody
    • HMT1 hnRNP methyltransferase like 1 antibody
    • hnRNP methyltransferase (S. cerevisiae) like 1 antibody
    • hnRNP methyltransferase like 1 antibody
    • Hrmt1l1 antibody
    • MGC111373 antibody
    • PRMT 2 antibody
    • PRMT2 alpha antibody
    • prmt2 antibody
    • PRMT2 beta antibody
    • PRMT2 gamma antibody
    • PRMT2 protein antibody
    • PRMT2L2 antibody
    • Protein arginine methyltransferase 2 antibody
    • Protein arginine N methyltransferase 2 antibody
    • Protein arginine N-methyltransferase 2 antibody
    • Zf2 antibody
    see all

Anti-PRMT2 antibody - ChIP Grade 图像



  • Predicted band size : 49 kDa
    Western blot analysis using whole HL60 cell lysate: PRMT2 (Arrow) was detected using ab3763. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
    Western blot analysis using whole HL60 cell lysate: PRMT2 (Arrow) was detected using ab3763. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  •  

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3763 to PRMT2 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 µl of ab3763 and 2x106 cells were used in each ChIP experiment.

  • ab3763 staining PRMT2 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PRMT2 antibody - ChIP Grade (ab3763)参考文献

This product has been referenced in:
  • Kitowska K  et al. Functional role and species-specific contribution of arginases in pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 294:L34-45 (2008). Read more (PubMed: 17934065) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LnCap Prostate Cancer)
Loading amount 10 µg
Specification LnCap Prostate Cancer
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jun 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"