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Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length human PRMT1.
Our Abpromise guarantee covers the use of ab12189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunocytochemsitry/Immunofluorescence analysis of MEFs labelling PRMT1 with ab12189. +/+ and PRMT1−/flox MEFs were treated with 2 μg/ml 4-hydroxytamoxifen (OHT) or untreated for 3 days. The cells were then seeded onto glass coverslips and cultured in fresh medium lacking OHT for 3 additional days. The cells were rinsed with ice cold methanol, fixed with 2% paraformaldehyde, and permeabilized with methanol -20°C for 5 minutes. The cells were first blocked for 20 minutes at room temperature (0.5% gelatin, 1× PBS) and then incubated with primary antibodies followed by washes (0.1% gelatin, 1% normal goat serum, 1X PBS) and incubation with Alexa Fluor 594-conjugated rabbit secondary antibody.