概述

  • 产品名称Anti-PRMT1抗体[EPR3292]
    参阅全部 PRMT1 一抗
  • 描述
    兔单克隆抗体[EPR3292] to PRMT1
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to residues in human PRMT1

  • 阳性对照
    • A549 cell lysate HeLa cell lysate NIH/3T3 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

Our Abpromise guarantee covers the use of ab92299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized secondary antibody is recommended as these have been found to give a stronger signal.

    WB: 1/5000 - 1/10000. Predicted molecular weight: 42 kDa.

    Is unsuitable for Flow Cyt, ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
    • 组织特异性Widely expressed.
    • 序列相似性Belongs to the protein arginine N-methyltransferase family.
    • 细胞定位Nucleus. Cytoplasm > cytosol.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANM 1 antibody
      • ANM1 antibody
      • ANM1_HUMAN antibody
      • HCP 1 antibody
      • HCP1 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
      • Histone-arginine N-methyltransferase PRMT1 antibody
      • HMT 2 antibody
      • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
      • HMT1 hnRNP methyltransferase antibody
      • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
      • HMT1 hnRNP methyltransferase like 2 antibody
      • HMT2 antibody
      • HRMT1 L2 antibody
      • HRMT1L 2 antibody
      • HRMT1L2 antibody
      • Human mRNA for suppressor for yeast mutant antibody
      • Human mRNA for suppressor for yeast mutant complete cds antibody
      • Interferon receptor 1 bound protein 4 antibody
      • Interferon receptor 1 bound protein4 antibody
      • Interferon receptor 1-bound protein 4 antibody
      • Interferon receptor 1bound protein 4 antibody
      • IR1 B4 antibody
      • IR1B 4 antibody
      • IR1B4 antibody
      • Mrmt 1 antibody
      • Mrmt1 antibody
      • PRMT 1 antibody
      • PRMT1 antibody
      • Protein arginine methyltransferase 1 antibody
      • Protein arginine N methyltransferase 1 antibody
      • Protein arginine N methyltransferase1 antibody
      • Protein arginine N-methyltransferase 1 antibody
      • R1B4 antibody
      • S. cerevisiae like 2 antibody
      see all

    Anti-PRMT1 antibody [EPR3292] 图像

    • All lanes : Anti-PRMT1 antibody [EPR3292] (ab92299) at 1/5000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH/3T3

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 42 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using ab92299 at 1/100 dilution.

    Anti-PRMT1 antibody [EPR3292] (ab92299)参考文献

    This product has been referenced in:
    • Sivakumaran H  et al. Overexpression of PRMT6 does not suppress HIV-1 Tat transactivation in cells naturally lacking PRMT6. Virol J 10:207 (2013). WB ; Human . Read more (PubMed: 23800116) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"