Anti-PRMT1抗体- ChIP Grade (ab3768)

概述

性能

应用

Our Abpromise guarantee covers the use of ab3768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 40 kDa.
ELISA 1/1000.
IHC-P 1/25 - 1/100.
ChIP Use 10µl for 106 cells.

靶标

  • 功能Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the protein arginine N-methyltransferase family.
  • 细胞定位Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANM 1 antibody
    • ANM1 antibody
    • ANM1_HUMAN antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
    • Histone-arginine N-methyltransferase PRMT1 antibody
    • HMT 2 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
    • HMT1 hnRNP methyltransferase antibody
    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
    • HMT1 hnRNP methyltransferase like 2 antibody
    • HMT2 antibody
    • HRMT1 L2 antibody
    • HRMT1L 2 antibody
    • HRMT1L2 antibody
    • Human mRNA for suppressor for yeast mutant antibody
    • Human mRNA for suppressor for yeast mutant complete cds antibody
    • Interferon receptor 1 bound protein 4 antibody
    • Interferon receptor 1 bound protein4 antibody
    • Interferon receptor 1-bound protein 4 antibody
    • Interferon receptor 1bound protein 4 antibody
    • IR1 B4 antibody
    • IR1B 4 antibody
    • IR1B4 antibody
    • Mrmt 1 antibody
    • Mrmt1 antibody
    • PRMT 1 antibody
    • PRMT1 antibody
    • Protein arginine methyltransferase 1 antibody
    • Protein arginine N methyltransferase 1 antibody
    • Protein arginine N methyltransferase1 antibody
    • Protein arginine N-methyltransferase 1 antibody
    • R1B4 antibody
    • S. cerevisiae like 2 antibody
    see all

Anti-PRMT1 antibody - ChIP Grade 图像



  • Predicted band size : 40 kDa
    Western blot analysis (whole HL60 cell lysate): PRMT1 (Arrow) was detected using ab3768 (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  •  
    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab4441 to Histone H3 (acetyl K9) and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE).
    10 µl of ab3768 and 2x10

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling PRMT1 - ChIP Grade with ab3768. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; heat mediated antigen retrieval was performed with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-PRMT1 antibody - ChIP Grade (ab3768)参考文献

This product has been referenced in:
  • Valentijn LJ  et al. Functional MYCN signature predicts outcome of neuroblastoma irrespective of MYCN amplification. Proc Natl Acad Sci U S A 109:19190-5 (2012). WB . Read more (PubMed: 23091029) »
  • Fathman JW  et al. NIP45 controls the magnitude of the type 2 T helper cell response. Proc Natl Acad Sci U S A 107:3663-8 (2010). WB ; Mouse . Read more (PubMed: 20133688) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (brain)
Specification brain
Blocking step Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2014

Application Western blot
Sample Mouse Cell lysate - whole cell (AML12 (mouse liver cell line))
Loading amount 30 µg
Specification AML12 (mouse liver cell line)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

提交于 May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (B cell line RS411)
Specification B cell line RS411
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control NR3C1 promoter binding site sequences
Negative control upstream sequences, IgG control IP
Username

Abcam user community

Verified customer

提交于 Jul 03 2012

I am very sorry for the delay in replying to you and for the problems you are having with those antibodies in ChIP. We have not tested in house those antibodies in ChIP and therefore it is very difficult to find what the problem might be. Ab540...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"