The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Use a concentration of 1 - 4 µg/ml.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Western blot - PRKRIR antibody (ab70334)
All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/ml
Lane 1 : whole cell lysate of HeLa cells at 50 µg Lane 2 : whole cell lysate of HeLa cells at 15 µg Lane 3 : whole cell lysate of HeLa cells at 5 µg Lane 4 : whole cell lysate of NIH3T3 cells at 50 µg
Predicted band size: 88 kDa Observed band size: 88 kDa
Immunoprecipitation - PRKRIR antibody (ab70334)
IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded.
Lane 1: ab70333 used at 3µg/ml lysate for IP.
Lane 2: ab70334 used at 3µg/ml lysate for IP.
Lane 3: IgG control.
ab70334 used at at 1µg/ml for the subsequent WB for all lanes.
Detection: Chemiluminescence with an exposure time of 30 seconds.