概述

  • 产品名称
    Anti-PRKCDBP抗体
    参阅全部 PRKCDBP 一抗
  • 描述
    兔多克隆抗体to PRKCDBP
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of human PRKCDBP

  • 阳性对照
    • A2058 cell lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: 1/1000.
    WB: 1/50 - 1/100. Predicted molecular weight: 28 kDa.
    Bands at 40-45 kDa may also occur due to 2 possible prenylation sites

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
    • 组织特异性
      Strongly expressed in mammary and epithelial cells.
    • 序列相似性
      Belongs to the PTRF/SDPR family.
    • 翻译后修饰
      In vitro, phosphorylated by PRKCD.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cavin 3 antibody
      • Cavin-3 antibody
      • CAVIN3 antibody
      • hSRBC antibody
      • MGC20400 antibody
      • PRDBP_HUMAN antibody
      • Prkcdbp antibody
      • Protein kinase C delta binding protein antibody
      • Protein kinase C delta-binding protein antibody
      • Sdr related gene product that binds to c kinase antibody
      • Serum deprivation response factor related gene product that binds to C kinase antibody
      • Serum deprivation response factor-related gene product that binds to C-kinase antibody
      • SRBC antibody
      see all

    图片

    • Anti-PRKCDBP antibody (ab76676) at 1/50 dilution + A2058 cell lysate at 35 µg

      Predicted band size : 28 kDa
      Observed band size : 29 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    This product has been referenced in:
    • Sathish V  et al. Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim Biophys Acta 1843:346-51 (2014). Read more (PubMed: 24275509) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Lung)
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册