RabMAb

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)抗体[EPR5671] (ab124709)

概述

  • 产品名称Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)抗体[EPR5671]
  • 描述
    兔单克隆抗体[EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • 特异性This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.

  • 阳性对照
    • HeLa cell lysates and Hek 293 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC/IF,IHC-P or IP.
  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] 图像

    • All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104, 112, 99 kDa
    • ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
      Green: untreated cells
      Red: Okadaic Acid + Calyculin A-treated cells
      Blue: untreated cells preincubated with the immunizing phosphopeptides
      Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .

    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)参考文献

    ab124709 has not yet been referenced specifically in any publications.

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