Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)抗体[EPR5671]
兔单克隆抗体[EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
Mouse, Rat, Human
Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.
- HeLa cell lysates and Hek 293 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF,IHC-P or IP.
Western blot - Anti-PRK3+PRK2+PRK1(phospho T718+T816+T774) (phospho T816 + T718 + T774) antibody [EPR5671] (ab124709)
All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.
Lysates/proteins at 10 µg per lane.
Predicted band size: 104, 112, 99 kDa
Bottom blot was probed with an anti-PNK2 antibody.
Flow Cytometry - Anti-PRK3+PRK2+PRK1(phospho T718+T816+T774) (phospho T816 + T718 + T774) antibody [EPR5671] (ab124709)
ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
Green: untreated cells
Red: Okadaic Acid + Calyculin A-treated cells
Blue: untreated cells preincubated with the immunizing phosphopeptides
Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"