Anti-Prion protein PrP抗体[1E5 / G6] (HRP) (ab3408)

概述

  • 产品名称Anti-Prion protein PrP抗体[1E5 / G6] (HRP)
    参阅全部 Prion protein PrP 一抗
  • 描述
    小鼠单克隆抗体[1E5 / G6] to Prion protein PrP (HRP)
  • 偶联物HRP
  • 特异性This antibody detects: Human recombinant prion protein, Bovine recombinant, native and PrPres proteinase K treated prion protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Recombinant bovine prion protein. Immunization was performed according to the protocol described by Hofmann, J. et al.

  • 表位This antibody has been shown to specifically bind between amino acids 140 - 160 of bovine prion protein, determined by pepscan (Osman, A.A. et al.).
  • 阳性对照
    • native bovine prion protein from brain (M. oblongata)

性能

应用

Our Abpromise guarantee covers the use of ab3408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at an assay dependent concentration (please check Abreview).
    WB: Use at a concentration of 1 - 10 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
    • 疾病相关Note=PrP is found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs.
      Defects in PRNP are the cause of Creutzfeldt-Jakob disease (CJD) [MIM:123400]. CJD occurs primarily as a sporadic disorder (1 per million), while 10-15% are familial. Accidental transmission of CJD to humans appears to be iatrogenic (contaminated human growth hormone (HGH), corneal transplantation, electroencephalographic electrode implantation, etc.). Epidemiologic studies have failed to implicate the ingestion of infected annimal meat in the pathogenesis of CJD in human. The triad of microscopic features that characterize the prion diseases consists of (1) spongiform degeneration of neurons, (2) severe astrocytic gliosis that often appears to be out of proportion to the degree of nerve cell loss, and (3) amyloid plaque formation. CJD is characterized by progressive dementia and myoclonic seizures, affecting adults in mid-life. Some patients present sleep disorders, abnormalities of high cortical function, cerebellar and corticospinal disturbances. The disease ends in death after a 3-12 months illness.
      Defects in PRNP are the cause of fatal familial insomnia (FFI) [MIM:600072]. FFI is an autosomal dominant disorder and is characterized by neuronal degeneration limited to selected thalamic nuclei and progressive insomnia.
      Defects in PRNP are the cause of Gerstmann-Straussler disease (GSD) [MIM:137440]. GSD is a heterogeneous disorder and was defined as a spinocerebellar ataxia with dementia and plaquelike deposits. GSD incidence is less than 2 per 100 million live births.
      Defects in PRNP are the cause of Huntington disease-like type 1 (HDL1) [MIM:603218]. HDL1 is an autosomal dominant, early onset neurodegenerative disorder with prominent psychiatric features.
      Defects in PRNP are the cause of kuru (KURU) [MIM:245300]. Kuru is transmitted during ritualistic cannibalism, among natives of the New Guinea highlands. Patients exhibit various movement disorders like cerebellar abnormalities, rigidity of the limbs, and clonus. Emotional lability is present, and dementia is conspicuously absent. Death usually occurs from 3 to 12 month after onset.
      Defects in PRNP are the cause of spongiform encephalopathy with neuropsychiatric features (SENF) [MIM:606688]; an autosomal dominant presenile dementia with a rapidly progressive and protracted clinical course. The dementia was characterized clinically by frontotemporal features, including early personality changes. Some patients had memory loss, several showed aggressiveness, hyperorality and verbal stereotypy, others had parkinsonian symptoms.
    • 序列相似性Belongs to the prion family.
    • 结构域The normal, monomeric form has a mainly alpha-helical structure. The disease-associated, protease-resistant form forms amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Disease mutations may favor intermolecular contacts via short beta strands, and may thereby trigger oligomerization.
      Contains an N-terminal region composed of octamer repeats. At low copper concentrations, the sidechains of His residues from three or four repeats contribute to the binding of a single copper ion. Alternatively, a copper ion can be bound by interaction with the sidechain and backbone amide nitrogen of a single His residue. The observed copper binding stoichiometry suggests that two repeat regions cooperate to stabilize the binding of a single copper ion. At higher copper concentrations, each octamer can bind one copper ion by interactions with the His sidechain and Gly backbone atoms. A mixture of binding types may occur, especially in the case of octamer repeat expansion. Copper binding may stabilize the conformation of this region and may promote oligomerization.
    • 翻译后修饰The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
      Isoform 2 is sumoylated by SUMO1.
    • 细胞定位Cell membrane. Golgi apparatus and Cytoplasm. Nucleus. Accumulates outside the secretory route in the cytoplasm, from where it relocates to the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alternative prion protein; major prion protein antibody
      • AltPrP antibody
      • ASCR antibody
      • CD230 antibody
      • CD230 antigen antibody
      • CJD antibody
      • GSS antibody
      • KURU antibody
      • Major prion protein antibody
      • p27 30 antibody
      • PRIO_HUMAN antibody
      • Prion protein antibody
      • Prion related protein antibody
      • PRIP antibody
      • PRNP antibody
      • PrP antibody
      • PrP27 30 antibody
      • PrP27-30 antibody
      • PrP33-35C antibody
      • PrPC antibody
      • PrPSc antibody
      • Sinc antibody
      see all

    Anti-Prion protein PrP antibody [1E5 / G6] (HRP) 图像

    • Anti-Prion protein PrP antibody [1E5 / G6] (HRP) (ab3408) at 1 µg/ml + native bovine prion protein from brain (M. oblongata)

      Performed under non-reducing conditions.

      Observed band size : 19 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 27 kDa (possible glycosylated form),30 kDa (possible glycosylated form).
    • ab3408 (1/100) staining Prion protein PrP (red) in inflammatory cells from peri-infarcted regions of a human brain after ischaemic stroke. Please refer to Abreview for further experimental details.

      See Abreview

    Anti-Prion protein PrP antibody [1E5 / G6] (HRP) (ab3408)参考文献

    This product has been referenced in:
    • Mitsios N  et al. Cellular prion protein is increased in the plasma and peri-infarcted brain tissue after acute stroke. J Neurosci Res 85:602-11 (2007). Read more (PubMed: 17149767) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
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    Abcam user community

    Verified customer

    提交于 Oct 23 2007

    Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab8999. As discussed on the telephone this afternoon please would you fill in the questionnaire by clicking on the link below. I would also appreciate it i...

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    Ab3408 has been tested in direct ELISA and Western Blot, but a concentration of 4 ng/ml is too low. The datasheet recommendations were for the unconjugated form of the antibody and I apologise for this mistake, it has immediately been corrected. I wo...

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    Thank you for your enquiry and your interest in our products. According to the datasheet, recombinant bovine prion protein was used to raise this antibody. It is suitable for Western blot and ELISA application which indicates that it recognizes both...

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